Ribonucleic Acid-dependent Ribonucleotide Incorporation
نویسندگان
چکیده
منابع مشابه
Ribonucleic Acid-dependent Ribonucleotide Incorporation
Several enzymatic reactions are known to utilize ribonucleoside triphosphates for incorporation of ribonucleotides into ribonucleic acid polymers. Two of these reactions have been well characterized. In one, cytidine 5’-phosphate and adenosine 5’-phosphate are added to the nucleoside terminal end of soluble RNA, forming an amino acid acceptor RNA terminated by the trinucleotide sequence, pCpCpA...
متن کاملRibonucleic Acid-dependent Ribonucleotide Incorporation. Ii. Inhibition of Polyriboadenylate Polymerase Activity following Bacteriophage Infection.
Synthesis of polyriboadenylate is accompanied by the concomitant release of inorganic pyrophosphate. Under appropriate conditions, poly Al is formed in excess of RNA added to the reaction mixture. The dependence of the reaction on RNA is shown (a) by complete loss of poly A synthesis in the presence of pancreatic RNase, although the reaction product is highly resistant to this RNase, and (b) by...
متن کاملIncorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations.
The incorporation of the cytokinin N(6)-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N(6)-benzyladenine-8-(14)C or N(6)-benzyladenine-8-(14)C: benzene-(3)H(G) has been examined. N(6)-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive N(6...
متن کاملCytoplasmic Incorporation of a Ribonucleic Acid Precursor in Amoeba proteus
The question of RNA synthesis in enucleate cytoplasm of Amoeba has been approached experimentally by incubating enucleate amoebae in a labelled RNA precursor and determining the incorporation into RNA autoradiographically. The results indicate that there is a cytoplasmic incorporation mechanism which can operate in the absence of the nucleus. A comparison is made between Acetabularia and Amoeba...
متن کاملUridine Nucleotide Incorporation into Pigeon Liver Microsome Ribonucleic Acid
The role of nucleotides as precursors of ribonucleic acid in animal tissues has been well documented (l-4). ilside from the formation of polymers by the enzyme polynucleotide phosphorylase (5), only one preliminary report of net synthesis of polyribonucleotides in cell-free systems has been made (6). Hilmoe and Heppel (7) have demonstrated the phosphorolysis of added polyadenylic acid by a part...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1962
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84523-4